Composite
Part:BBa_K571008
Designed by: Lu-Chu Ke Group: iGEM11_TzuChiU_Formosa (2011-10-05)
R0011+acsAB+acsCD/pSB1C3
The biobrick consists of complete acs operon gene and a promoter which will activate the operon when it is induced by IPTG, cellulose synthase will be synthesized, which later use to produce cellulose.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal EcoRI site found at 7892
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal PstI site found at 4802
Illegal PstI site found at 4988
Illegal PstI site found at 5438
Illegal PstI site found at 7274 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal EcoRI site found at 7892
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal PstI site found at 4802
Illegal PstI site found at 4988
Illegal PstI site found at 5438
Illegal PstI site found at 7274
Illegal NotI site found at 3233 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal EcoRI site found at 7892
Illegal BglII site found at 1222 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal EcoRI site found at 7892
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal PstI site found at 4802
Illegal PstI site found at 4988
Illegal PstI site found at 5438
Illegal PstI site found at 7274 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 666
Illegal EcoRI site found at 2067
Illegal EcoRI site found at 7892
Illegal PstI site found at 822
Illegal PstI site found at 1218
Illegal PstI site found at 2058
Illegal PstI site found at 4802
Illegal PstI site found at 4988
Illegal PstI site found at 5438
Illegal PstI site found at 7274
Illegal NgoMIV site found at 2441
Illegal NgoMIV site found at 2481
Illegal NgoMIV site found at 5321
Illegal NgoMIV site found at 6397
Illegal NgoMIV site found at 7517
Illegal AgeI site found at 4613
Illegal AgeI site found at 5533
Illegal AgeI site found at 6824 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1155
[edit]
Categories
Parameters
None |